细胞生物学及实验(何九军) - 中国大学mooc

同步更新 19
Introduction of Molecular cell biology

Cell theory-test

1、What did Leeuwenhoek do?
    A、Looked at cork
    B、Stated that plants are made of cells
    C、Stated that animals are made of cells
    D、Used simple light microscope and saw microorganisms



2、He discovered that all animals were made of cells, which contributed to the development of the cell theory:
    A、A.van Leeuwenhoek
    B、Robert Hooke
    C、Zacharias Jansse
    D、Theodor Schwann



3、Who was the first person to see cells under the microscope and give them a name?
    A、A.van Leeuwenhoek
    B、Robert Hooke
    C、Theodor Schwann
    D、Zacharias Jansse



4、Which of the following is NOT one of the main components of the cell theory?
    A、cells must contain DNA
    B、all living things are made of cells
    C、cells can only come from other cells
    D、cells are the basic unit of life



Biomembrane Structure

Biomembrane Structure-test

1、All the following statements describe biomembranes except:
    A、Different biomembranes may contain different proportions of the same phospholipids.
    B、The two leaflets of a biomembrane may contain different phospholipids.
    C、Some biomembranes have free edges.
    D、Some phospholipids and cholesterol may cluster to form lipid rafts.



2、The major site of lipid synthesis in eukaryotic cells is the
    A、Nucleus
    B、Endoplasmic reticulum
    C、Mitochondria
    D、Cell membrane



3、Phospholipids with short or unsaturated fatty acyl chains
    A、decrease membrane fluidity.
    B、increase membrane fluidity.
    C、cause biomembranes to become thicker
    D、allow hydrophilic molecules to diffuse across the lipid bilayer.



4、The _______ are transmembrane proteins.
    A、lipid-anchored membrane proteins
    B、integral membrane proteins
    C、peripheral membrane proteins
    D、extracellular matrix proteins



5、Glycolipids and glycoproteins are especially abundant in the
    A、nucleus.
    B、mitochondrial inner membrane
    C、cytosol
    D、plasma membrane



6、Phosphate containing lipids include all of the following except
    A、phospholipids.
    B、sphingolipid
    C、triglyceride
    D、phosphatidylinositol



7、A lipid bilayer is the fundamental structural component of all cell membranes.



8、Maintenance of the lipid bilayer in the plasma membrane requires special enzymes and the hydrolysis of ATP.



9、The most abundant lipids in cell membranes are ________________.



10、Sugar-containing lipids called ____________ are found only in the outer half of the bilayer and their sugar groups are exposed ac the cell surface.



Culturing and visualizing cells

Culturing and visualizing cells-test

1、The most direct source of monoclonal antibodies are ________ cells.
    A、B lymphocytes
    B、T lymphocytes
    C、myeloma
    D、Hybridoma



2、The fluorescent properties of dyes such as FITC can provide information on the
    A、location of specific proteins.
    B、concentration of ions in specific regions of the cell.
    C、the amount of DNA in a cell.
    D、volume of a cell.



3、What factors necessary for growth of animal cells in culture are provided by serum?
    A、amino acids
    B、precursors of DNA synthesis
    C、growth factors
    D、vitamins



4、What is the advantage of using GFP-tag to visualize specific proteins, instead of staining cells with fluorescent dyes that bind to the target protein?
    A、GFP fusions enable one to track changes in the location of the protein in living cells; staining usually requires fixed cells
    B、GFP fusions enable higher resolution than staining with fluorescent probes.
    C、GFP permits the position of the protein in the cell more precisely than fluorescent probes
    D、GFP fusions are not subject to artifacts; fluorescent probes may introduce background artifacts.



5、Which one cleaves pre-miRNA in the cytoplasm to produce miRNA?
    A、Dicer
    B、RISC
    C、shRNA
    D、siRNA



6、A single monoclonal antibody can bind to several different sites on an antigen



7、RNAi can be mediated just by introducing the already processed miRNA/siRNA into cells



8、___________________ degrades mRNAs with sequences complementary to a bound siRNA



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